(별표 3)

실험동물연구지원센터 미생물 모니터링 검사항목

검사 방법	Microbes	Mouse	Rat
혈청검사	Mouse cytomegalovirus (MCMV)	○
	Reovirus 3	○	○
	Rotavirus (Epizootic diarrhea of infant mice)	○
	Sendai virus (HVJ)	○	○
	Mouse hepatitis virus (MHV)	○
	Ectromelia virus (Mouse pox)	○
	Mycoplasma spp.	○
	Clostridium piliforme (Tyzzer's disease)	○	○
	Mouse adenovirus (MAV)	○	○
	Hanta virus (Hemorrhagic fever with renal syndrome)	○	○
	Pneumonia virus of mice (PVM)	○	○
	Mouse encephalomyelitis virus (GD Ⅶ)	○	○
	Minute virus of mice (MVM)	○
	Lymphocytic choriomeningitis (LCM) virus	○
	Cilia associated respiratory (CAR) bacillus	○
	Sialodacryoadenitis (SDA) virus		○
	Kilham rat virus		○
	H-1 virus		○
배양검사	Pasteurella pneumotropica	○	○
	Salmonella spp.	○
	E.colo O002a,dK(B)	○
	Corynebacterium kutscheri	○	○
	Pseudomonas aeruginosa	○	○
	Staphylococcus aureus	○
	Bordetella bronchiseptica		○
	Streptococcus pneumoniae		○
	Salmonella typhimurium		○
	Dermatophytes	○	○
검경	Giardia muris	○	○
	Spironucleus muris	○	○
	Syphacia soo.	○
	Aspiculuris tetraptera	○
	Trichomonas spp.		○
	Syphacia spp.		○
	Entamoeba spp.		○
	Other intestinal protozoa	○
	Ectoparasite	○
PCR	Helicobacter hepaticus	○
	Helicobacter bilis	○

검역대상 병원균의 목록

병원체	마우스	랫드
Corynebacterium kuscheri Bacillus piliformis Mycoplasma pulmonis Pseudomonas aeruginosa Pasteurella multocida Bordetella bronchiseptica Streptococcus zooepidemicus Salmonella spp. Brucella canis Leptospira icterohamorrhagiae Microsporum canis Shigella spp. Mycobacterium tuberculosis Sendai virus Mouse hepatitis virus Feline viral rhinotrachitis virus Feline panleukopenia virus B virus Rabies virus Eimeria spp. Toxoplasma gondii Entameba histolytica Dirofilaria immitis Endoparasites Pasoroptea cuniculi Sialodacryoadenitis virus	○ ○ ○ ○ ○ ○	○ ○ ○ ○ ○

모니터링 대상 미생물

Pathogen		Mouse	Rat
Virus	Ectromelia virus Epizootic diarrhea of infant mice virus (EDIM) Hantavirus H-1 virus K virus (Mouse pneumonitis virus) Kilham rat virus (KRV) Lactate dehydrogenase-elevating virus Lymphocytic choriomeningitis virus (LCMV) Minute virus of mice (MVM) Mouse adenovirus (MAV) Mouse cytomegalovirus (MCMV) Mouse encephalomyelitis virus Mouse hepatitis virus (MHV) Mouse parvovirus (MPV) Mouse thymic virus (MTV) Pneumonia virus of mice (PVM) Polyomavirus Rat coronavirus (Sialodacryoadenitis virus) Rat parvovirus (RPV) Reovirus 3 Sendai virus	o o o x o x o o o o o o o o o o o x x o o	x x o o x o x o x o x o x x x o x o o o o
Bacteria	Bordetella bronchiseptica Cilia-associated respiratory (CAR) bacillus Citrobacter rodentium Clostridium piliforme (Tyzzer's organism) Corynebacterium kutscheri Helicobacter species Klebsiella species Mycoplasma species Pasteurella species Pseudomonas species Salmonella species Staphylococcus aureus Streptobacilus moniliformis Streptococcus species	o o o o o o o o o o o o o o	o o x o o o o o o o o o o o
	Ectoparasites Intestinal protozoa Pinworm	o o o	o o o
Fungus	Dermatophytes Encephalitozoon cuniculi Pneumocystic carinii	o o o	o o o

모니터링 검사방법

병원체	검사방법	검사재료	비고
Bacteria
Pseudomonas aeruginosa	배양	맹장내용물, 피부, 장기의 농양	NAC abar
Salmonella spp	배양	맹장내용물, 장내용물, 간, 비장내 병소	DHL agar, Hajna
Escherichia coli O115a,c:K(B)	배양	Cecal cont.	DHL agar
Pasteurella pneumotropica	배양	인후두, 기관	Horse blood agar
Bordetella bronchiseptica	배양, 혈청검사	비강, 기관, 폐병소	DHL agar Agglutination
Corynebacterium kutscheri	배양, 혈청검사	맹장내용물, 구강, 간, 비장, 신장내 병변	FNC agar
Tyzzer's organism	혈청검사 현미경검사	간과 심장의 괴사성 병소	CF, Giemsa stain
Mycoplasma spp.	배양 혈청검사	비강, 기관, 폐병소	PPLO agar, CF, ELISA
Virus
Sendai virus (HVJ)	혈청검사		CF, HI, ELISA
Mouse hepatitis virus (MHV)	혈청검사		CF, ELISA
Sialodacryoadenitis virus (SDA)	혈청검사		CF(antigen:MHV)
Ectromelia virus	혈청검사		HI(antigen:Vaccinia)
Hemorrhagic fever with renal syndrome (HFRS)	혈청검사		ELISA, IAHA, IFA
Protozoa
Eimeria spp.	현미경검사	맹장내용물
Giardia muris	현미경검사	십이지장내용물
Hexamita muris	현미경검사	십이지장내용물
Parasites
Syphacia spp.	현미경검사	항문	egg
Myobia musculi	현미경검사	피부병변	Mite egg

참고1. 마우스와 랫드의 번식에 있어서 병원체 검사를 위한 단위

	Sample size		Testing/animal
Sampling frequency	Age	No. animals	Sero.	Bact.	Para.	Path.
Every 3 months (For mouse, rat)	Weaning	>2	-	+	+	+
	10-14 weeks (young adults)	>4	+	+	+	+
	>6 months (retired breeders)	>4	+	+	+	+
Every 6 months (For Rabbits)	12-14 weeks (young adults)	>4	+	+	+	+
	>6 months (retired breeders)	>4	+	+	+	+

참고2.  각 단체 또는 판매자들이 정한 마우스와 랫드의 검사대상 병원체

Examination items	SNU		FELASA		ICLAS		Fackson Lab		Charles River
	Mice	Rat	Mice	Rat	Mice	Rat	Mice	Rat	Mice	Rat
Serology Panel		●		●		●				●
SDAV, RCV	●		●		●		●		●
MHV	●	●	●	●	●	●	●		●
Sendai virus	●	●	●	●	●	●	●		●	●
Mycolasma Pulmonis	●	●	●	●	●	●	●		●	●
Crynebacterium kuscheri
Clostridium pilifome	●	●	●	●	●	●	●		●	●
KRV, RPV, H-1		●		●						●
EDIM	●		●				●		●
MVM, MPV	●		●		●	●	●		●
PVM	●		●	●	●	●	●		●	●
REO-3	●	●	●	●	●	●	●		●	●
GD-VII	●								●	●
LCM	●		●		●	●	●		●	●
CAR Bacillus					●	●	●		●	●
Hantaan virus		●	●	●			●		●	●
Encephalitozoon cuniculi	●	●							●	●
K virus	●		●				●		●
Mouse Adenovirus	●	●	●		●	●	●		●	●
Ectromelia	●		●		●	●	●		●
Polyoma	●		●				●		●
MCMV	●		●				●		●
RCMV										●
Thymic Virus			●				●
LDHV			●		●	●	●
PARV NS1									●
MTLV									●
TMEV			●	●			●
Bacterial Panel
Bordetella bronchiseptica	●	●	●	●			●		●	●
Beta-hemolytic Streptococcus			●	●	●	●			●	●
Citrobacter freundii			●						●	●
Corynebacterium kutscheri	●	●	●	●	●	●	●		●	●
Leptospira			●	●
Klebsiella oxytoca					●	●	●		●	●
Klebsiella pneumotropica							●		●	●
Pasteurella pneumotropica	●	●			●	●	●		●	●
Pasteurella multocida							●
Pseudomonas aeruginosa	●	●			●	●	●		●	●
Salmonella sp	●	●	●	●	●	●	●		●	●
Staphylococcus aureus	●	●			●	●	●		●	●
streptococcus moniliformis							●
streptococcus pneumonia			●	●			●		●	●
Pasteurella sp.			●	●					●	●
Streptobacillus moniliformis			●	●			●		●	●
Helicobacter sp.	●	●			●	●	●		●	●
Dermatophytes
Examination items	SNU		FELASA		ICLAS		Fackson Lab		Charles River
	Mice	Rat	Mice	Rat	Mice	Rat	Mice	Rat	Mice	Rat
Parasites
Artropod ectoparasites	●	●	●	●			●		●	●
Helminth endoparasites	●	●	●	●	●	●	●		●	●
Enteric protozoans	●	●	●	●	●	●	●		●	●
Hemoprotozoans(Rickettsia)

Charles River: 2003. 4.2 charles river diagnostics report 참고

ANZCCART: ANIMAL HEALTH MONTORING IN THE RESEARCH ENVIRONMENT Notes from the Workshop

This was ANZCCART' s third workshop for 1998 참고

SLC: Japan SLC 실험동물 - 중앙실험동물(주) report 참고

FELASA: Report of the Federation of European Laboratory Animal Science Association(FELASA) Working Group on Animal

Health accepted by the FELASA Board of Management November 1992

Samtako: 2000. 1~3월 Samtako Health Profile Report 참고

ICLAS: 2003 Lab. Animal Mdgazine 32 참고

Jackson Lab: 2003 Jaxmice. jax. org/health 참고

표 2. 계속

Examination items	ANZCCART		Santako		SLC
	Mice	Rat	Mice	Rat	Mice	Rat
Serology Panel
SDAV, RCV		●		●		●
MHV	●		●		●
Sendai virus	●	●	●	●	●	●
Mycolasma Pulmonis	●	●	●	●	●	●
Crynebacterium kuscheri				●	●
Clostridium pilifome			●	●	●	●
KRV, RPV, H-1		●		●
EDIM	●		●
MVM, MPV	●		●
PVM		●	●	●	●	●
REO-3	●	●	●	●
GD-VII	●		●	●
LCM	●	●	●	●	●	●
CAR Bacillus	●	●	●	●
Hantaan virus	●	●	●	●
Encephalitozoon cuniculi	●	●	●	●
K virus	●		●
Mouse Adenovirus	●	●	●		●	●
Ectromelia	●		●		●
Polyoma	●		●
MCMV	●		●
RCMV
Thymic Virus			●
LDHV			●
PARV NS1
MTLV	●
TMEV
Bacterial Panel
Bordetella bronchiseptica	●	●	●	●		●
Beta-hemolytic Streptococcus	●	●	●	●
Citrobacter freundii	●		●	●
Corynebacterium kutscheri	●	●	●	●	●	●
Leptospira			●	●
Klebsiella oxytoca	●	●	●	●
Klebsiella pneumonia	●	●	●	●
Pasteurella pneumotropica	●	●	●	●	●	●
Pasteurella multocida	●	●
Pseudomonas aeruginosa	●	●	●	●	●	●
Salmonella sp	●	●	●	●	●	●
Staphylococcus aureus	●	●	●	●
streptococcus moniliformis	●	●	●	●
streptococcus pneumonia	●	●	●	●		●
streptococcus zooepidemicus
Pasteurella sp.	●	●
Streptobacillus moniliformis	●	●
Helicobacter sp.	●	●
Examination items	ANZCCART		Santako		SLC
	Mice	Rat	Mice	Rat	Mice	Rat
Dermatophytes			●	●
Parasites
Artropod ectoparasites	●	●	●	●	●	●
Helminth endoparasites	●	●	●	●	●	●
Enteric protozoans	●	●	●	●	●	●
Hemoprotozoans(Rickettsia)			●	●

SNU: 2003 Laboratory Animal Medicine, College of Veterinary Medicine, SNU 실시예